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View Article Google Scholar Altogether distinct clonotype protein sequences were encoded by the antigen-specific clonotype nucleotide sequences from all 5 donors. Table shows the number of antigen-specific clonotypes identified by dextramer-based MIRA from a replicate experiment using a mixed sample containing PBMCs from 3 donors donors 1, 2 and 4. All possible pairs were assessed from each of the 5 donors and the fraction of clonotypes identified in one individual and found in another individual was plotted. B Plot of all antigen-specific clonotypes identified from donor 1 between time points spaced two months left and seven years apart right. We observed that the Flu peptide antigen tested elicited a higher proportion of shared clonotypes than those specific to CMV and EBV peptide antigens. Mixing PBMCs from different donors.
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